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Single nucleotide polymorphisms of 17β‐hydroxysteroid dehydrogenase type 7 gene: Mechanism of estramustine‐related adverse reactions?
Author(s) -
Ozeki Takeshi,
Takeuchi Megumi,
Suzuki Motofumi,
Kitamura Tadaichi,
Takayanagi Risa,
Yokoyama Haruko,
Yamada Yasuhiko
Publication year - 2009
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2009.02374.x
Subject(s) - single nucleotide polymorphism , allele , gene , microbiology and biotechnology , transfection , luciferase , du145 , transcription (linguistics) , lncap , medicine , biology , genetics , genotype , prostate cancer , cancer , linguistics , philosophy
Objectives: To investigate the influence of single nucleotide polymorphisms (SNP) on transcription of the 17β‐hydroxysteroid dehydrogenase (HSD17B7) gene. Methods: Luciferase reporter genes containing a 5′‐flanking of the HSD17B7 gene, as well as the sequence around the SNP, were transfected into LNCaP and DU145 cells. Then, luciferase assays were carried out. Results: The presence of the G allele resulted in an increase of transcriptional activity derived from the 5′‐flanking region of the HSD17B7 gene by 270% and 370% in LNCaP and DU145 cells, respectively. Transcriptional activity of the HSD17B7 gene containing the G allele was higher than that of the C allele. Conclusions: The transcriptional activity of the HSD17B7 gene containing the G allele is higher than that of the C allele. This difference in HSD17B7 expression may regulate the risk of peripheral edema as an adverse reaction induced by estramustine phosphate sodium.