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Type‐I interferon receptor expression: Its circadian rhythm and downregulation after interferon‐α administration in peripheral blood cells from renal cancer patients
Author(s) -
Shiba Masahiro,
omura Norio,
Nakai Yasutomo,
Nakayama Masashi,
Takayama Hitoshi,
Inoue Hitoshi,
Tsujimura Akira,
Nishimura Kazuo,
Okuyama Akihiko
Publication year - 2009
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2009.02265.x
Subject(s) - medicine , peripheral blood mononuclear cell , downregulation and upregulation , circadian rhythm , interferon , renal cell carcinoma , endocrinology , immunology , biology , in vitro , biochemistry , gene
Objectives: To investigate the regulation of interferon‐α (IFN‐α) receptor expression in metastatic renal cell carcinoma (RCC) after IFN‐α administration. Methods: Blood sampling was carried out in eight patients with metastatic RCC and six healthy volunteers. Flow‐cytometric analysis using a monoclonal antibody against the active subunit of the type‐I IFN‐α receptor (IFNAR2) was carried out to examine the circadian rhythm of IFNAR2 expression in peripheral blood mononuclear cells (PBMC) as well as its downregulation after IFN‐α administration. Results: According to its circadian rhythm IFNAR2 in PBMC had a peak expression at night. Once IFN‐α is administered, IFNAR2 levels in PBMC showed downregulation within 48 h and recovered within another 48 h. Conclusions: Our findings might support the establishment of an optimal schedule for IFN‐α administration.