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Role of endogenous cannabinoids in ischemia/reperfusion injury following testicular torsion in rats
Author(s) -
Beheshtian Azadeh,
Salmasi Amirali Hassanzadeh,
Payabvash Seyedmehdi,
Kiumehr Saman,
Nezami Behtash Ghazi,
Rahimpour Sina,
Rabani Razieh,
Tavangar Seyed Mohammad,
Dehpour Ahmad Reza
Publication year - 2008
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2008.02018.x
Subject(s) - malondialdehyde , superoxide dismutase , lipid peroxidation , apoptosis , medicine , reperfusion injury , endocrinology , tunel assay , testicular torsion , chemistry , ischemia , oxidative stress , biochemistry , surgery
Objectives: The role of endogenous cannabinoids in ischemia/reperfusion induced germ cell apoptosis in rats was investigated. Methods: Baseline group was for basal normal values. The Sham operated group served as a control group. The torsion/detorsion (T/D) group underwent torsion (1 h) and detorsion; AN1, AN2, and AN3 groups received anandamide (10 mg/kg) 30 min before torsion, 30 min after torsion, and just after detorsion, respectively. In the AM251 group, AM251 (0.5 mg/kg) was injected 45 min before torsion and in the AN/AM group, AM251 and anandamide were injected 45 and 30 min before torsion, respectively. Lipid peroxidation, antioxidant enzymes, and germ cell apoptosis was determined. Results: Malondialdehyde (MDA) levels in the T/D group were significantly higher than the control group. Moreover, MDA values in the AN1, AN2, and AN3 groups were significantly lower than T/D. There were significant decreases in catalase and superoxide dismutase activities in the T/D group versus the control group. These values in the AN1, AN2, and AN3 groups were significantly higher than T/D. It was also shown that MDA levels in the AN/AM group were significantly higher than the AN1 group. In the AN/AM group, catalase and superoxide dismutase activities were significantly lower versus the AN1 group. The mean germ cell apoptosis scores in all animals with testicular T/D were significantly higher than the control group. There was no difference between the apoptotic indices in the AN1, AN2, AN3, and T/D groups. Apoptosis scores in AM251 and AN/AM were significantly higher compared with the T/D and AN1 groups. Conclusions: Although anandamide increased antioxidant markers, it failed to reduce germ cell apoptosis. AM251 worsened the antioxidant defense system, which is reflected as higher germ cell apoptosis.