Premium
Tumor‐infiltrating lymphocytes derived from human renal cell carcinoma: Clonal analysis of its characteristics
Author(s) -
Shimabukuro Tomoyuki,
Naito Katsusuke
Publication year - 2008
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2007.01977.x
Subject(s) - clone (java method) , cytotoxic t cell , tumor infiltrating lymphocytes , renal cell carcinoma , cd8 , medicine , cancer research , cytotoxicity , population , immunology , biology , oncology , immune system , in vitro , genetics , gene , environmental health
Aim: To assess the characteristics of activated tumor‐infiltrating lymphocytes (TIL), we report the isolation, growth response, and functional analysis of a CD4 ‐ CD8 + TIL‐clone derived from human renal cell carcinoma (RCC). Methods: Bulk TILs were expanded from a human RCC and the lymphocytes were separated into a CD8 + enriched population. Subsequently, using the limiting dilution technique, a TIL clone was established and its growth response, phenotype and cytotoxic activity were analyzed. Results: A clone, T16‐13, by day 94 numbering 1 × 10 7 cells, was harvested and characterized as a CD4 ‐ CD8 + clone. On day 144, the cytotoxic activity of this clone against the autologous tumor was relatively high (2.3 ± 0.7 LU 30 /10 6 cells). Meanwhile, against allogeneic renal tumors, there was no cytotoxic activity (−0.1 LU 30 /10 6 cells). Conclusions: A TIL clone possessing modest autologous tumor‐specific cytotoxicity can be isolated from human RCC. The characteristics analysis of various TIL clones may provide a better understanding of an RCC tumor microenvironment and may help to establish new modalities for the treatment of patients with metastatic kidney cancer.