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EDTA‐induced urothelial cell shedding for the treatment of superficial bladder cancer in the mouse
Author(s) -
NATIV OFER,
DALAL EILATA,
HIDAS GUY,
ARONSON MOSHE
Publication year - 2006
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2006.01541.x
Subject(s) - medicine , urothelial cancer , saline , urology , urothelial cell , bladder tumor , bladder cancer , cell , pathology , urinary bladder , urothelium , cancer , chemistry , biochemistry
Aim: The aim of this study was to determine the effect of intravesical EDTA instillation on the development of intravesically implanted tumor cells in normal mice. Methods: The mouse bladder tumor (MBT‐2) model was used in female C3H/eb mice to evaluate the amount of normal urothelial cell shedding, and the degree of tumor growth inhibition following intravesical EDTA instillation in comparison with phosphate‐buffered saline (PBS) instillation. Results: At 1 h after instillation, the number of urothelial cells aspirated was 500–1000 per PBS‐treated mouse and 10 000–20 000 per EDTA‐treated mouse ( P < 0.00001). The bladder weight, which reflected the effect of the agent on the tumor, was similar in the untreated and PBS‐treated mice (105.46 ± 46 mg and 106.2 ± 50 mg, respectively). It was significantly lower in the EDTA‐treated mice (80.4 ± 42 mg) ( P = 0.0045). Conclusions: Intravesical administration of EDTA results in significant normal and neoplastic urothelial cell shedding. Intravesical irrigation with EDTA may prevent adherence of the malignant cells to the bladder wall following tumor resection.