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Fibronectin inhibits endocytosis of calcium oxalate crystals by renal tubular cells
Author(s) -
TSUJIHATA MASAO,
YOSHIMURA KAZUHIRO,
TSUJIKAWA KOZO,
TEI NORIHIDE,
OKUYAMA AKIHIKO
Publication year - 2006
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2006.01396.x
Subject(s) - endocytosis , calcium oxalate , fibronectin , extracellular matrix , extracellular , adhesion , calcium , microbiology and biotechnology , biophysics , biochemistry , medicine , chemistry , cell , biology , organic chemistry
Background: Fibronectin (FN; 230 kDa) is a multifunctional α2‐glycoprotein distributed throughout the extracellular matrix and body fluids. We recently reported that FN has a protective effect against injury of renal tubular cells by exposure to oxalate and calcium oxalate (CaOX) crystals and inhibits the adhesion of CaOX crystals to renal tubular cells. In the study presented here, we investigated whether FN has inhibitory effect on crystal endocytosis by renal tubular cells. Methods: The inhibitory effect of FN on endocytosis of CaOX crystals by MDCK cells was examined by using a radioactivity uptake assay. Also, crystal endocytosis by cells was morphologically assessed by means of transmission electron microscopy (TEM). Results: FN had inhibitory effects on CaOX crystal endocytosis by MDCK cells. The morphological TEM study showed that few crystals were taken into cells when FN was added compared to the number of crystals when FN was not added. Conclusion: We found that FN had the inhibitory effects on the interaction between crystals and renal tubular cells, including the adhesion or endocytosis of crystals by cells.