Premium
Exogenous human recombinant interleukin‐10 protects the kidney against hypoxia‐induced renal injury in immature rats
Author(s) -
ÖZTÜRK HAYRETTIN,
ONEN ABDURRAHMAN,
ÖZTÜRK HULYA,
BUYUKBAYRAM HUSEYIN
Publication year - 2006
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2006.01304.x
Subject(s) - medicine , malondialdehyde , hypoxia (environmental) , necrosis , kidney , endocrinology , tumor necrosis factor alpha , recombinant dna , renal injury , oxidative stress , biology , oxygen , chemistry , biochemistry , organic chemistry , gene
Aim: The purpose of this study was to determine the effects of exogenous human recombinant interleukin‐10 (rhIL‐10) on hypoxia‐induced renal injury in immature rats. Method: The study was performed on 1‐day‐old Sprague–Dawley rat pups. Group 1 ( n = 8) served as non‐hypoxic controls. Group 2 (untreated, n = 8) rats were subjected to hypoxia‐reoxygenation (H/O) and were then returned to their mothers. Group 3 (rhIL‐10 treated, n = 8) rats were subjected to H/O, were returned to their mothers, and were treated with rhIL‐10 (75 µg/kg subcutaneously) for the next 3 days. All animals were killed on day 4 and renal specimens were obtained to determine the tissue level of malondialdehyde (MDA) and histological changes. Results: In the untreated group, moderate or severe renal tubular necrosis was observed However, the tubular necrosis score was significantly less in the rhIL‐10 treated rats than in the untreated rats ( P < 0.05). In the untreated group, MDA levels were significantly increased compared with the control and rhIL‐10 groups ( P < 0.001 and P < 0.05, respectively). In the rhIL‐10 treated group, MDA levels were not significantly different compared with the control group. Conclusion: RhIL‐10 has a protective effect against hypoxia‐induced renal injury in immature rats by depression of tissue MDA level and renal tubular necrosis score.