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Real‐time quantitative analysis for human telomerase reverse transcriptase mRNA and human telomerase RNA component mRNA expressions as markers for clinicopathologic parameters in urinary bladder cancer
Author(s) -
TAKIHANA YOSHIO,
TSUCHIDA TAKAYUKI,
FUKASAWA MIZUYA,
ARAKI ISAO,
TANABE NOBUAKI,
TAKEDA MASAYUKI
Publication year - 2006
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2006.01300.x
Subject(s) - telomerase reverse transcriptase , telomerase , bladder cancer , messenger rna , rna , cancer research , reverse transcription polymerase chain reaction , medicine , real time polymerase chain reaction , reverse transcriptase , microbiology and biotechnology , pathology , biology , cancer , gene , genetics
Aim: The expression of the telomerase subunits such as human telomerase reverse transcriptase (hTERT) and human telomerase RNA component (hTR) may be associated with tumor development and progression. We evaluated the relationship between mRNA quantification of both hTERT and hTR and clinicopathologic parameters in bladder cancer. Methods: We examined the mRNA expression of hTERT and hTR in 29 specimens with bladder cancer (Grade: Grade I, 9 cases; Grade II, 13 cases and Grade III, 7 cases. Stage: pTa‐pT1, 18 cases; pT2‐T4, 11 cases). We immediately froze all of specimens obtained during TUR‐Bt and isolated the total RNA from each specimen. We measured the quantity of hTERT, hTR and GAPDH mRNA by a real‐time reverse transcription–polymerase chain reaction method based on TaqMan technology. Results: The hTERT/GAPDH mRNA ratio and hTERT mRNA/total RNA in superficial bladder tumor was significantly lower than in invasive bladder tumor. The hTR/GAPDH mRNA ratio and hTR mRNA/total RNA in superficial tumor were significantly lower than in invasive bladder tumor. The hTERT mRNA expression significantly correlated with tumor grade, but the hTR mRNA expression did not correlate with tumor grade. There was no significant difference in the hTERT/GAPDH mRNA ratio and hTR mRNA/total RNA according to multiplicity of bladder tumor. Conclusions: Our results demonstrated that the expression of hTERT mRNA correlated with the progression of stage and grade in bladder cancer. The quantitative analysis of hTERT and hTR mRNA might be a marker for clinicopathologic parameters in bladder cancer.