Premium
Effect of ascorbic acid treatment on endothelium‐dependent and neurogenic relaxation of corpus cavernosum from middle‐aged non‐insulin dependent diabetic rats
Author(s) -
GÜR SERAP,
KARAHAN S TUNA,
ÖZTÜRK BÜLENT,
BADILLI MEHMET
Publication year - 2005
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2005.01125.x
Subject(s) - ascorbic acid , endocrinology , medicine , diabetes mellitus , insulin , acetylcholine , nitric oxide synthase , nitric oxide , stimulation , cholinergic , endothelium , chemistry , food science
Aim:The purpose of the present study was to investigate functional responses and nitric oxide synthase activity in the corpus cavernosum of young control, middle‐aged control and middle‐aged non‐insulin dependent diabetic rats.Methods:The animal groups were treated with ascorbic acid.Results:Acetylcholine‐mediated endothelium‐dependent relaxation of cavernosal tissue was significantly attenuated from a maximum of 58.0 ± 4.1% (1 mmol, n = 10) in young rats to 44.3 ± 1.6% in aged‐matched controls (P < 0.05) and to 23.3 ± 2.8% in non‐insulin‐dependent diabetes mellitus rats (P < 0.01). These deficits in acetylcholine responsiveness were completely prevented by ascorbic acid treatment. Non‐adrenergic non‐cholinergic relaxations evoked by electrical field stimulation (0.5–64.0 Hz) in the corpus cavernosum of middle‐aged control and non‐insulin dependent (NID) diabetic rats are blunted and were not restored by ascorbic acid. The histochemical ndings demonstrated a decrease of nicotinamide adenine dinucleotide phosphate‐diaphorase staining in the cavernosal tissue obtained from middle‐aged control rats and middle‐aged diabetic rats.Conclusion:Partial correction by ascorbic acid may suggest the importance of reactive oxygen species and a therapeutic approach in impotent NID diabetic men.