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Effects of platelet‐activating factor on nitrergic transmission in rabbit corpus cavernosum
Author(s) -
ÖZGER SEVIL,
BARUN SÜREYYA,
VURAL İSMAIL MERT,
ERCAN ZEYNEP SEVIM,
SARİOĞLU YUSUF
Publication year - 2005
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2005.01109.x
Subject(s) - sodium nitroprusside , phenylephrine , nitric oxide , carbachol , medicine , platelet activating factor , endocrinology , endothelium , vasodilation , stimulation , blood pressure
Abstract Aim: The information currently available suggests that nonadrenergic noncholinergic (NANC) transmitters, particularly nitric oxide, are involved in the relaxation of penile erectile tissues. Platelet‐activating factor (PAF) is a chemical mediator and is involved in many physiological and pathophysiological events. It is well known that several of the vascular actions of PAF are mediated by the generation of nitric oxide. We designed this study to test the hypothesis that PAF has an effect on NANC responses in rabbit corpus cavernosum strips. Methods: Rabbit corpus cavernosum strips were precontracted with phenylephrine (10 −5 mol/L). Isometric tension changes produced by carbachol (10 −9 −10 −5 mol/L), sodium nitroprusside (10 −8 −10 −5 mol/L) and electrical field stimulation (for 10 s at sequential frequencies of 2, 4, 8, 16, and 32 Hz as square‐wave pulses of 50 mV) were recorded with a pressure transducer. These relaxations were compared to those obtained in the presence of PAF. Results: PAF had no effect on endothelium‐dependent, endothelium‐independent or electrical field stimulation‐induced NANC relaxation responses in isolated rabbit corpus cavernosum strips. There was no statistically significant difference between the pD 2 and E max values for carbachol or sodium nitroprusside in the presence of PAF. Conclusions: Our results suggest that PAF does not modify the endothelium‐dependent, endothelium‐independent or electrical field stimulation‐induced NANC relaxation responses in isolated rabbit corpus cavernosum strips.