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Determination of gelatinase A using a modified indirect hemagglutination assay in human prostate cancer screening and assessment of its correlation with prostate‐specific antigen parameters
Author(s) -
KHORRAMIZADEH MOHAMMAD REZA,
AALIZADEH NASTARAN,
PEZESHKI MOHAMMAD,
GHAHARY AZIZ,
ZERAATI HOJAT,
BERAHMEH AZAR,
SAFA OMID,
SAADAT FARSHID
Publication year - 2005
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2005.01094.x
Subject(s) - medicine , titer , prostate cancer , prostate specific antigen , gelatinase a , prostate , gelatinase , cancer , hyperplasia , antigen , hemagglutination , gastroenterology , urology , immunology , antibody , matrix metalloproteinase
Background: Prostate cancer is the most common malignancy affecting men and is a major cause of cancer death. There are increasing data on novel tumor markers, such as gelatinase A, which play a key role in tissue invasion and metastasis. Objectives: We designed a study to evaluate total gelatinase A content using a simple and applicable Indirect hemagglutination (IHA) test in harmony with gelatinase A activity in serum samples as compared with prostate‐specifc antigen (PSA) parameters. Methods: In this study, we analysed the circulating form of gelatinase A (MMP‐2) in patients suffering from either benign prostate hyperplasia (n = 54) or prostate cancer (n = 26) versus normal individuals as control (n = 26). The gelatinolytic activity was determined by zymography and total MMP‐2 content was measured by a novel IHA method. Total PSA and free PSA were quantified using a standard ELISA technique. Results: Correlation of densitometric analysis of gelatinase A activity and IHA titer is significant at the 0.01 level (P < 0.01, rho = 0.916). Correlation of PSA and IHA titer is significant at the 0.01 level (P < 0.01, rho = 0.746). Correlation of free PSA and IHA titer is significant at the 0.01 level (P < 0.01, rho = 0.749). Borderline of IHA titer in patients with prostate cancer was 512 ± 1 tube titer, in benign prostate hyperplasia patients was 128 ± 1 tube titer and the titer in normal individuals was 8 ± 1 tube titer. Conclusions: These results demonstrate that assessment of gelatinase A might be a promising procedure for monitoring and screening patients with prostate cancer.