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Oxalate‐degrading Providencia rettgeri isolated from human stools
Author(s) -
HOKAMA SANEHIRO,
TOMA CLAUDIA,
IWANAGA MASAAKI,
MOROZUMI MAKOTO,
SUGAYA KIMIO,
OGAWA YOSHIHIDE
Publication year - 2005
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2005.01083.x
Subject(s) - oxalate , microbiology and biotechnology , enterobacteriaceae , bacteria , blot , gel electrophoresis , biochemistry , polyacrylamide gel electrophoresis , chemistry , biology , escherichia coli , enzyme , gene , genetics , organic chemistry
Background: Oxalate‐degrading bacteria are thought to metabolize intestinal oxalate and thus decrease the urinary excretion of oxalate by reducing its intestinal absorption. Methods: We have isolated several novel oxalate‐degrading bacteria from human stools. Oxalate degrading bacteria were investigated to characterize their protein profiles with antibodies against oxalyl‐coenzyme A decarboxylase (65 kDa) and formyl‐coenzyme A transferase (48 kDa) purified from Oxalobacter formigenes . Results: One of these isolates was identified as Providencia rettgeri , which showed two proteins (65 kDa and 48 kDa) on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) that were not found in non‐oxalate‐degrading P. rettgeri . Antibodies reacted with the 65 and 48 kDa proteins from the P. rettgeri strain on Western blotting. An Oxalobacter formigenes formyl‐coenzyme A transferase gene probe reacted with chromosomal DNA from P. rettgeri on Southern blotting under high stringency conditions, while an Oxalobacter formigenes oxalyl‐coenzyme A decarboxylase gene probe did not react under the same conditions. Conclusions: The mechamism of oxalate degradation by P. rettgeri appears to be similar to that of Oxalobacter formigenes . This is the first report of a facultative oxalate‐degrading organism that is one of the Enterobacteriaceae.