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Global analysis of gene expression profiles in ileum in a rat bladder augmentation model using cDNA microarrays
Author(s) -
MIYAKE HIDEAKI,
HARA SHOJI,
ETO HIROSHI,
KAMIDONO SADAO,
HARA ISAO
Publication year - 2004
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2004.00942.x
Subject(s) - ileum , complementary dna , microarray , gene expression , dna microarray , microarray analysis techniques , gene , gene expression profiling , urinary bladder , reverse transcription polymerase chain reaction , medicine , microbiology and biotechnology , biology , endocrinology , genetics
Abstract Background: The objective of the present study was to globally characterize the changes in the gene expression profile in the ileum after long‐term urine exposure in a rat ileal augmented bladder model using cDNA microarrays. Methods: Bladder augmentation using the ileum was performed in female 8‐week‐old rats. The ileal epithelia used for bladder augmentation were harvested 1 and 3 months postoperatively and changes in the gene expression in these tissues were compared with that of intact ileal epithelia from sham‐operated rats using cDNA microarrays consisting of 1176 rat genes. Results: Marked changes in gene expression in the ileum used for bladder augmentation were observed for 30 genes (16 up‐regulated and 14 down‐regulated genes). The differentially expressed genes include those associated with signal transduction, cell adhesion and stress response. Subsequent evaluation of changes in two randomly selected genes from the 30 differentially expressed genes by semiquantitative reverse transcription‐polymerase chain reaction demonstrated the reliability of the present cDNA microarray analyses. Conclusion: The present experiments identified an extensive list of genes differentially expressed in the ileum after bladder augmentation, providing valuable information for the pathophysiological assessment of patients who undergo urinary reconstruction and representing a source of novel targets for treating complications after urinary diversion.