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Increased expression of lung resistance‐related protein in lower grade urothelial carcinoma of the renal pelvis and ureter
Author(s) -
KONG CHUIZE,
ZENG YU,
WU XIUXIAN,
LI JIAQING,
ZHU YUYAN,
CHEN YAN
Publication year - 2004
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.2004.00874.x
Subject(s) - renal pelvis , ureter , urothelium , medicine , multiple drug resistance , malignancy , messenger rna , immunohistochemistry , carcinoma , cancer research , urothelial carcinoma , pathology , multidrug resistance associated protein 2 , lung , gene , urology , urinary bladder , biology , drug resistance , cancer , bladder cancer , atp binding cassette transporter , biochemistry , microbiology and biotechnology , transporter
Background: Lung resistance‐related protein ( LRP ), like multidrug resistance gene 1 ( MDR1 ) and multidrug resistance‐associated proteins ( MRP ), has been associated with intrinsic therapeutic resistance in various malignancies. To date, there has been no study on the expression of LRP in urothelial carcinomas of the renal pelvis and ureter. We investigated the protein and mRNA expression levels of LRP , MDR1 and MRP1 in this malignancy and the clinical significance of their expression was evaluated. Methods: Forty urothelial carcinomas of the renal pelvis and ureter and 31 normal upper urothelial samples were examined by immunohistochemistry and reverse transcription polymerase chain reaction to determine the protein and mRNA levels of the multidrug resistance‐related genes, respectively. Results: The positive staining rates and mRNA levels of LRP were the highest among these multidrug resistance‐related genes in both normal urothelium and carcinoma examinations. In contrast to the up‐regulated expression of MDR1 , the expression of LRP tended to be down‐regulated in carcinomas. Moreover, the expression of LRP inversely correlated with tumor grades, but this correlation was not found for the other two genes. However, there was no correlation among the expression of the three genes observed. Conclusion: Lung resistance‐related protein was strongly expressed in urothelial carcinomas of the renal pelvis and ureter, particularly in well‐differentiated carcinomas.