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Renal Tubular Apoptosis after Release of Ureteral Obstruction in the Rat Kidney
Author(s) -
Tanji Nozomu,
Yokoyama Masayoshi,
Terada Nobuyuki,
Shudo Masachika,
Takeuchi Ken,
Takeuchi Masafumi
Publication year - 1998
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.1998.tb00600.x
Subject(s) - apoptosis , agarose gel electrophoresis , dna fragmentation , hydronephrosis , medulla , kidney , medicine , fragmentation (computing) , renal cortex , electron microscope , pathology , agarose , gel electrophoresis of nucleic acids , microbiology and biotechnology , anatomy , dna , programmed cell death , biology , biochemistry , urinary system , ecology , physics , optics
Background: information concerning the mechanisms underlying recovery from hydronephrosis is limited. The frequency of apoptosis during healing from hydronephrosis was studied using a rat kidney model. Methods: The presence of apoptosis was studied using an in situ DNA 3 end labeling method, electron microscopy, and agarose gel electrophoresis. Results: The degree of apoptosis in both the medulla and cortex gradually increased during ureteral obstruction as shown by in situ DNA 3 end labeling. Release of the ureteral obstruction resulted in a further increase in the degree of apoptosis in the medulla and cortex. The increase in apoptosis in the medulla was transient and lasted for only 4 days following release, while that in the cortex continued for at least 3 weeks. Apoptosis in the glomerulus was not observed. Electron microscopy revealed cells with aggregated chromatin in compact granular masses that abutted the nuclear membrane. Following release of ureteral obstruction, DNA fragmentation characteristic of apoptosis was visible on agarose gel electrophoresis. Conclusion: These results suggest that apoptosis is involved in post‐obstructive tubular damage in the rat kidney.