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Spontaneous Loss of Antibiotic‐Resistant Plasmids Transferred to Escherichia coli in Experimental Chronic Bladder Infection
Author(s) -
Inoue Yoshihiro
Publication year - 1997
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.1997.tb00191.x
Subject(s) - plasmid , escherichia coli , microbiology and biotechnology , antibiotics , bacteria , plasmid preparation , antibiotic resistance , biology , medicine , dna , gene , genetics , pbr322
Background We investigated whether Escherichia coli that has been transformed with antibiotic‐resistant plasmids spontaneously loses the plasmids while infecting the rat bladder. Methods A nontransmissible antibiotic‐resistant plasmid was transferred to a clinically isolated strain of E. coli. A knotted thread holding the strain was implanted into the bladder of male Wistar rats. One or 4 weeks later, the total number of bacteria and that of plasmid‐carrying bacteria were measured in the bladder. Results When plasmid‐carrying E. coli alone was inoculated, most of the plasmid‐carrying E. coli were replaced with plasmid‐free E. coli. When plasmid‐carrying and plasmid‐free E. coli were simultaneously inoculated, this replacement was accelerated. Conclusion These results suggest that E. coli carrying antibiotic‐resistant plasmids spontaneously lose the plasmids as time passes, and thus become sensitive to antibiotics. Int J Urol 1997;4:285‐288