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ANALYSIS OF nm23 GENE EXPRESSIONS IN HUMAN BLADDER AND RENAL CANCERS
Author(s) -
Kanayama Hiroomi,
Takigazva Hiroshi,
Kagazva Susumii
Publication year - 1994
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.1994.tb00058.x
Subject(s) - northern blot , bladder cancer , metastasis , medicine , stage (stratigraphy) , pathology , urinary bladder , pathological , cell , kidney , messenger rna , gene expression , cancer , gene , cancer research , biology , paleontology , biochemistry , genetics
We measured nm23‐H1 and nm23‐H2 mRNA levels in tissues from 22 human bladder cancers and 16 renal cell carcinomas, and in 7 bladder cancer and 6 renal cancer cell lines by Northern blot and slot blot hybridization analyses. Differences in mRNA levels were evaluated in primary tumor tissues and in paired normal tissues and cell lines. Moreover, nrh23 gene expression in primary tumor tissues was compared with clinicopathological features. High nm23‐H1 and nm23‐H2 expression was observed in cancerous areas of human bladder tissue ( nm23‐H1 : p = 0.001, nm23‐H2 : p = 0.001) and bladder cancer cell lines ( nm23‐H1: p = 0.001, nm23‐H2: p< 0.001) compared with that in normal bladder mucosa. However, mRNA levels of both nm23 genes were not associated with histological grade, pathological stage, tumor metastasis or prognosis. On the other hand, in human renal cell carcinomas, levels of both nm23 mRNAs in tumor tissues were similar to those in paired normal kidneys, but elevated in cultured cell lines ( nm23‐H1: p= 0.002, nm23‐H2: p = 0.014). Moreover, there was a tendency towards high nm23 gene expression in grade 2 tumors compared with grade 1 (grade 1 vs grade 2, nm23‐H1: p = 0.107, nm23‐H2: p = 0.008; no grade 3 tumors in this study) and in high stage renal cancers (stage II vs stage III, nm23‐H1: p = 0.023, nm23‐H2: p = 0.005). From these results, we suggest that reduced nm23 mRNA levels are not associated with metastasis of either bladder or renal cancers and there may be some tissue‐specific differences in the expression patterns of nm23‐H1 and nm23‐H2 in human cancers.