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ISOLATION AND PURIFICATION OF A HIGH MOLECULAR WEIGHT SUBSTANCE FROM HUMAN URINE WHICH INHIBITS CALCIUM OXALATE CRYSTAL GROWTH
Author(s) -
Morikawa Mitsuru,
Yachiku Sunao,
Arima Shigeru
Publication year - 1994
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1111/j.1442-2042.1994.tb00038.x
Subject(s) - calcium oxalate , urine , oxalate , polyacrylamide gel electrophoresis , chromatography , uronic acid , fast protein liquid chromatography , inhibitory postsynaptic potential , biochemistry , enzyme , medicine , chemistry , endocrinology , polysaccharide , organic chemistry
A high molecular weight inhibitor of calcium oxalate crystal growth in human urine was investigated. Three inhibitors were isolated by DEAE‐Sephacel ion‐exchange chromatography and, of these, the substance we named Peak 3 protein seemed to be the main inhibitor in human urine. Peak 3 protein was purified by fast protein liquid chromatography and polyacrylamide gel electrophoresis. This substance, with a molecular weight of 30 kDa, did not contain uronic acid and its inhibitory activity decreased after digestion with proteinase. The difference between Peak 3 protein and several inhibitors previously reported was investigated but no clear difference could be found. The fact that it was the protein structure which was responsible for the inhibitory activity and the fact that Peak 3 protein probably possessed many side‐chains which did not contribute to the inhibitory activity influenced the outcome of the investigation.