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Lack of an association between E‐selectin gene polymorphisms and risk of Kawasaki disease
Author(s) -
Shirakawa Toshihiko,
Ikeda Kazuyuki,
Nishimura Shinji,
Kuniba Hideo,
Nakashima Kazuhisa,
Motomura Hideki,
Mizuno Yumi,
Zaitsu Masafumi,
Nakazato Mio,
Maeda Takahiro,
Hamasaki Yuhei,
Hara Toshiro,
Moriuchi Hiroyuki
Publication year - 2012
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.2012.03608.x
Subject(s) - medicine , single nucleotide polymorphism , allele , kawasaki disease , snp , coronary artery disease , allele frequency , gastroenterology , polymorphism (computer science) , gene , genotype , genetics , artery , biology
Background: Coronary artery lesions (CAL) are a serious complication of Kawasaki disease (KD). The increased serum E‐selectin level during the acute phase of KD and the association of E‐selectin gene ( SELE ) polymorphisms with the prevalence of coronary artery disease in adults suggest a possible association between SELE polymorphisms and the development of CAL in KD patients. Methods: The subjects consisted of 177 KD patients, including 59 with and 118 without CAL, and 305 healthy controls. Two single nucleotide polymorphisms (SNP) of SELE , 98G>T (rs1805193) and Ser128Arg (rs5361), were genotyped by direct sequencing and the high‐resolution melting curve method, respectively. The allele distributions were assessed using the chi‐squared test. Results: There were no significant differences in the T allele frequency at 98G>T between KD patients and controls (1.4% vs 1.0%, P = 0.55) or between KD patients with and without CAL (1.7% vs 1.3%, P = 0.77). Similarly, there were no differences in the distribution of the C allele (128Arg) at Ser128Arg between KD patients and controls (4.5% vs 3.4%, P = 0.40) or between KD patients with and without CAL (4.2% vs 4.7%, P = 0.86). Conclusion: Although no association was detected between these SELE polymorphisms and the prevalence of KD or the development of CAL, this may have been due to the study limitations, including a low frequency of the minor alleles and a small sample size. A larger‐scale association study is needed in order for a definitive conclusion to be made as to whether these SNP are associated with susceptibility to KD or not.