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In vitro production of bilirubin photoisomers by light irradiation using neoBLUE
Author(s) -
OKADA HITOSHI,
ABE TAE,
ETOH YOKO,
YOSHINO SHUJI,
KATO IKUKO,
IWAKI TAKUMA,
OKUBO KENSUKE,
YASUDA SANEYUKI,
KAWADA KOU,
KUSAKA TAKASHI,
NAMBA MASANORI,
NISHIDA TOMOKO,
IMAI TADASHI,
ISOBE KENICHI,
ITOH SUSUMU
Publication year - 2007
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.2007.02379.x
Subject(s) - bilirubin , irradiation , blue light , medicine , serum bilirubin , direct bilirubin , chemistry , biochemistry , materials science , optoelectronics , gastroenterology , enzyme , physics , alkaline phosphatase , nuclear physics
Background: The light‐emitting diode is used as one of the new light sources for phototherapy. NeoBLUE (Atom Medical, Tokyo, Japan) incorporates blue light‐emitting diodes for the treatment of neonatal hyperbilirubinemia. The authors compared the in vitro efficacy of neoBLUE with conventional phototherapy devices. Methods: The three light devices used included neoBLUE and two conventional phototherapy devices with six blue–white (BW) or six green (GR) fluorescent tubes. A bilirubin/human serum albumin solution (15 mg/dL) in 200 × 300 mm elliptical bag was irradiated with each three light device. The average light intensity of neoBLUE, BW and GR was 22.5, 10.2 and 2.6 μW/cm 2 per nm, respectively, for the irradiated area. Bilirubin photoisomers and native bilirubin were measured by high‐performance liquid chromatography. Results: In neoBLUE, BW and GR, the respective production rate of cyclobilirubin was 6.0, 3.7 and 3.9 × 10 −2 mg/dL/min, and the respective (4Z, 15E)‐bilirubin/(4Z, 15Z)‐bilirubin ratio after irradiation was 0.44, 0.33 and 0.12; the (4Z, 15Z)‐bilirubin reduction rate at 20 min after irradiation was 60, 68 and 82%, respectively. The reduction rate of (4Z, 15Z)‐bilirubin correlated with the (4Z, 15E)‐bilirubin/(4Z, 15Z)‐bilirubin ratio. Conclusion: Phototherapy using the neoBLUE under high level may be clinically more effective than therapy using the conventional light source from the results of the production rate of cyclobilirubin.