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Successful polymerase chain reaction‐based diagnosis of fungal meningitis in a patient with chronic granulomatous disease
Author(s) -
Tsuge Ikuya,
Makimura Koichi,
Natsume Jun,
Kubota Toshiko,
Hasegawa Shinji,
Kawabe Takashi,
Nakashima Sachie,
Aso Kosaburo,
Negoro Tamiko,
Watanabe Kazuyoshi
Publication year - 1998
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1998.tb01947.x
Subject(s) - chronic granulomatous disease , medicine , polymerase chain reaction , meningitis , cerebrospinal fluid , antifungal , differential diagnosis , immunology , complication , microbiology and biotechnology , pathology , gene , dermatology , surgery , biology , genetics
Meningitis is not a common complication of chronic granulomatous disease (CGD). Here, we present details of a 3‐year‐old boy with X‐linked CGD, who suffered from fungal meningitis. While 19 samplings using conventional cerebrospinal fluid (CSF) cultures failed to detect any organisms, fungal DNA was identified in the CSF by a new polymerase chain reaction (PCR)‐based method. The patient recovered without any sequelae after treatment with a combination of antifungal agents, interferon‐φ and granulocyte infusions. This case report demonstrates that fungal meningitis must be included in the differential diagnosis of infections in CGD patients and that the PCR‐based detection of fungal DNA is a powerful tool for diagnosis.