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Epstein‐Barr virus infection, Hodgkin's disease, non‐Hodgkin's lymphoma, and reactive follicular hyperplasia in Japanese children: Evaluation of paraffin‐embedded specimens using polymerase chain reaction and immunohistochemistry
Author(s) -
HIRAIWA HISAYUKI,
HAMAZAKI MINORU,
MURATA HIROAKI,
TANIGUCHI KIYOSU,
SAKURAI MINORU
Publication year - 1997
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1997.tb03574.x
Subject(s) - follicular hyperplasia , medicine , lymphoma , mononucleosis , lymphoid hyperplasia , virus , pathology , epstein–barr virus , polymerase chain reaction , immunohistochemistry , lymph node , reed–sternberg cell , biopsy , virology , biology , hodgkin lymphoma , gene , biochemistry
Abstract Epstein‐Barr virus (EBV) infections are common in Japanese children, with infections by EBV type 1. The relationships between EBV infection and lymphadenopathies in Hodgkin's disease (HD), non‐Hodgkin's lymphomas (NHL), reactive follicular hyperplasia (RFH), and infectious mononucleosis (IM) in 37 Japanese children were evaluated. Formalin‐fixed, paraffin‐embedded lymph node specimens that were obtained at surgical resection or biopsy were evaluated for the presence of EBV DNA and the latent membrane protein‐1 (LMP‐1) using polymerase chain reaction (PCR) and immunohistochemical staining. The PCR detected EBV DNA in nine of 13 (69.2%) patients with RFH, including a case of IM, all three (100%) patients with HD, and one of 21 (4.8%) patients with NHL. All EBV‐positive samples contained EBV type 1. Reed‐Sternberg's cells in HD were immunohistochemically positive for LMP‐1, whereas all cases of RFH and NHL were negative for LMP‐1. Results suggest that EBV infection may be related to HD. Although no proof exists that EBV infection contributes to the transformation of cells, thus causing RFH or NHL, the present authors suggest that the EBV‐positive cases in Japanese children demonstrate a relationship between the clinical and histopathological features of the lymphadenopathy and EBV‐type 1 infection.