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Detection of human immunodeficiency virus‐1 DNA, RNA and antibody, and occult blood in inactivated saliva: Availability of the filter paper disk method
Author(s) -
KAKIZAWA JUNKO,
USHIJIMA HIROSHI,
OKA SHINICHI,
IKEDA YUSEI,
SCHRÖDER HEINZ C,
MÜLLER WERNER EG
Publication year - 1996
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1996.tb03473.x
Subject(s) - medicine , saliva , virology , occult , human immunodeficiency virus (hiv) , antibody , dna , rna , filter paper , immunology , gene , pathology , genetics , chromatography , alternative medicine , biology , chemistry
Eighty three samples of saliva were collected from 60 subjects who were asymptomatic carriers of AIDS related complex (ARC) and AIDS. They included hemophiliacs, homosexuals and heterosexuals. Occult blood, human immunodeficiency virus‐1 (HIV‐1) antibody and DNA were assayed after heat treatment at 56°C by strip method, particle agglutination and polymerase chain reaction (PCR), respectively. HIV‐1 RNA was assayed by reverse transcription (RT)‐PCR after heat treatment at 56°C or 90°C, or after application to filter paper disks with drying and heat treatment at 90°C. Positive results were found in 53% (occult blood), 73% (HIV‐1 antibody), 23% (HIV‐1 DNA, 56°C), 34% (HIV‐1 RNA, 56°C), 33% (HIV‐1 RNA, 90°C) and 25% (HIV‐1 RNA, 90°C, filter paper disk), respectively. The results did not depend on the stage of the disease or the route of infection. Other studies have reported the use of the filter paper disk method to detect antibodies against HIV and HIV genomes in blood samples. The present study demonstrated that it could also be used for the detection of HIV genomes in saliva samples.