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Effects of cytokines on hematopoietic progenitor cells in cord blood, in bone marrow, and in peripheral blood mobilized by chemotherapy and G‐CSF
Author(s) -
URASHIMA MITSUYOSHI,
HOSHI YASUTAKA,
AKIYAMA MASAHARU,
KAMIJO MAKOTO,
SHISHIKURA AKIHIRO,
KATO YOKO,
AKATSUKA JUNICHI,
MAEKAWA KIHEI
Publication year - 1995
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1995.tb03679.x
Subject(s) - haematopoiesis , bone marrow , medicine , progenitor cell , stem cell factor , peripheral blood mononuclear cell , granulocyte colony stimulating factor , granulocyte , erythropoietin , immunology , stem cell , chemotherapy , microbiology and biotechnology , biology , in vitro , biochemistry
We compared the effects of various combinations of cytokines (stem cell factor [SCF], interleukin [IL] −3, IL‐6, granulocyte‐colony stimulating factor [G‐CSF], erythropoietin [EPO]) among the growth of human hematopoietic progenitor cells from cord blood (CB), bone marrow (BM), and peripheral blood mononuclear cells (MNC) mobilized by chemotherapy and G‐CSF (PB) in a semi‐solid medium. Macroscopic colonies, that were visible to the naked eye, were formed from PB‐MNC within 1 week even without cytokines. They consisted of blasts containing macrophage‐like cells with immature nuclei on Wright stain, and were strongly accelerated by IL‐3. Macroscopic colonies were also formed from CB‐MNC. However, they appeared after 1–3 weeks and synergistic effects of SCF with other cytokines, especially EPO, were prominent. Macroscopic colonies were not formed from BM‐MNC. Granulocyte‐colony stimulating factor was effective in increasing colony forming units of granulocyte macrophage from BM‐MNC and they appeared between 1 and 2 weeks. These results suggested that the quality of hematopoietic progenitor cells was different among blood sources. This might lead to different bone marrow recovery patterns after transplantation of each blood source. The appropriate cytokines should be added to evaluate their exact potential.