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Ex vivo expansion of umbilical cord blood hematopoietic progenitor cells by combinations of cytokines
Author(s) -
URASHIMA MITSUYOSHI,
HOSHI YASUTAKA,
AKIYAMA MASAHARU,
KAMIJO MAKOTO,
ATO YOKO K,
AKATSUKA JUNICHI,
MAEKAWA KIHEI,
MATSUZAKI MASAHARU,
OHNISHI JUN,
KAIHARA CHIHARU
Publication year - 1995
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1995.tb03289.x
Subject(s) - medicine , ex vivo , haematopoiesis , progenitor cell , umbilical cord , progenitor , immunology , in vivo , stem cell , microbiology and biotechnology , biology , genetics
Ex vivo expansion of hematopoietic progenitor cells in the umbilical cord blood mononuclear cells (CB‐MNC) was investigated in liquid culture system with various combinations of cytokines (stem cell factor [SCF], interleukin [IL]‐3, IL‐6, granulocyte‐colony stimulating factor [G‐CSF], erythropoietin [EPO], and interferon [INF]‐γ). Non‐lineage‐committed hematopoietic progenitor cells and lineage committed hematopoietic progenitor cells were represented as CD34 + CD38 − and CD34 + CD38 + subpopulations, respectively. Although absolute CD34 + CD38 − cell numbers decreased even in the presence of multicytokines, the combinations of SCF plus IL‐6 and SCF plus IL‐3 plus IL‐6 plus INF‐γ were significantly effective in maintaining CD34 + CD38 − cells than the other combinations ( P < 0.05). After 4 weeks of culture, CD34 + CD38 − cells disappeared in all combinations of cytokines. Absolute CD34 + CD38 + cell numbers increased in the presence of cytokines. Maximal expansion of CD34 + CD38 + cells were observed in the combinations of SCF plus IL‐3 plus IL‐6 plus EPO (19.8 ± 3.3 ‐fold) and SCF plus IL‐3 plus IL‐6 plus G‐CSF (18.3 ± 2.6). The combination of SCF plus IL‐3 plus IL‐6 was also effective to expand CD34 + CD38 + cells (15.8 ± 3.9). However, the expansion was transient and they decreased to zero within 3 weeks. In the combinations of SCF plus IL‐6 and SCF plus IL‐3 plus IL‐6 plus INF‐γ, maximal expansion was inferior to the others but CD34 + CD38 + cells were maintained more than 4 weeks. These results suggested that the indication of CBT can be expanded into older children by ex vivo augmentation of CB hematopoietic progenitor cells using multi‐cytokines.