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A flow cytometric method for measurement of hydrogen peroxide generation by pediatric polymorphonuclear leukocytes stimulated by Staphylococcus aureus and Escherichia coli
Author(s) -
IHARA TOSHIAKI,
YAMAWAKI KATSUHIRO,
FUJIWARA TAKASHI,
KITAMURA KENJI,
SAKURAI MINORU,
KAMIYA HITOSHI
Publication year - 1994
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1994.tb03172.x
Subject(s) - staphylococcus aureus , hydrogen peroxide , escherichia coli , medicine , microbiology and biotechnology , reactive oxygen species , bacteria , granulocyte , immunology , biochemistry , biology , genetics , gene
The generation of hydrogen peroxide (H 2 O 2 ), one of the reactive oxygen species, by polymorphonuclear leukocytes (PMN) stimulated by Staphylococcus aureus and Escherichia coli was studied in infants by cytofluorography. After heparhized whole blood was incubated with bacteria for 60 min, generated H 2 O 2 was measured. The positive rate of H 2 O 2 generation of PMN and mean fluorescent intensity of positive PMN stimulated by S. aureus and E. coli were significantly reduced in infants aged < 1 year and H 2 O 2 generation increased with advancing age. In 10–15 year old children, the level of generated H 2 O 2 reached adult levels. When sera from 1 year old children were added to separated PMN from healthy adults, H 2 O 2 generation was reduced. In contrast, H 2 O 2 generation by PMN from 1 year old children was increased by the addition of adult sera. These results suggest that the ability to generate H 2 O 2 in response to S. aureus and E. coli is lower in infants and that such reduced activity may be related to the susceptibility of such infants to S. aureus and E. coli infections.