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Effect of Serum pH on Bilirubin‐Protein Binding
Author(s) -
Yeung C.Y.,
Lee FT.,
Wong H.N.
Publication year - 1992
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1992.tb00920.x
Subject(s) - titration , medicine , bilirubin , sephadex , horseradish peroxidase , albumin , peroxidase , chromatography , serum albumin , titration curve , serum bilirubin , size exclusion chromatography , biochemistry , enzyme , chemistry , inorganic chemistry
Progressive increase of the serum pH upon standing and storage in different conditions is documented. Bilirubin albumin titration studies were performed on serum of different pH by Sephadex gel filtration and horseradish peroxidase oxidation methods. Results showed that different pH produced different titration curves and that more reproducible and consistent results were obtained with the peroxidase oxidation titration method. Addition of HC1 and CO 2 to the serum also produced different bilirubin‐protein titration results. For blirubin albumin binding studies, we suggest to adjust the pH to 7.4 with CO 2 to obtain clinically meaningful data.