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Possible Role of Streptococcus pyogenes in Mucocutaneous Lymph Node Syndrome VI. Heightened Cellular Reactivity to Streptolysin‐O in Mice Infected with S. pyogenes and in MCLS Patients
Author(s) -
Akiyama Takehisa,
Osawa Nobutaka,
Yamaura Noboru,
Yasahiro Kimio,
Hiraishi Satoshi
Publication year - 1988
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1988.tb02499.x
Subject(s) - streptococcus pyogenes , medicine , streptolysin , mucocutaneous zone , immunology , lymph node , microbiology and biotechnology , in vitro , toxic shock syndrome , streptococcus , etiology , disease , pathology , biology , bacteria , bacterial protein , biochemistry , genetics , staphylococcus aureus
The present investigations were performed to determine, by using the macro‐phage migration inhibition test (MIT), whether cell‐mediated hypersensitivity to streptolysin‐O (SLO) was developed in mice infected with Streptococcus pyogenes as well as in patients with mucocutaneous lymph node syndrome (MCLS), with the following results: 1. The splenic lymphocytes from murine hosts infected with B‐346 op strain of S. pyogenes , which fails to induce cellular immunity in infected mice, showed a complete lack of responsiveness to SLO. In contrast, the mice infected with streptococci of Sv and S‐43 strains, both of which possess an intrinsic activity of eliciting enhanced cellular hypersensitivity in the same species of animals, exhibited positive responses to the hemolysin from strep‐tococcal culture supernatant. 2. Although a considerable number of MCLS patients in the acute phase of the illness failed to respond to SLO, a complete restoration of their responsiveness was observed in parallel with recovery from the acute illness. Conversely, all patients in the subacute phase of the disease reacted well in vitro to SLO, followed by a gradual decline of their responsiveness over a period of 1–2 weeks. Furthermore, the implications of these findings are discussed in association with the possible role of S. pyogenes as an etiological agent for MCLS.