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Release of High Molecular Weight Neutrophil Chemotactic Activity from Human Cultured Basophilic Cells
Author(s) -
Nagakura Toshikazu,
Shichijo Kozaburo,
Likura Yoji,
Kobayashi Miyuki,
Saito Hirohisa,
Otani Takeshi
Publication year - 1987
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1987.tb00356.x
Subject(s) - basophilic , chemotaxis , histamine , medicine , immunoglobulin e , in vitro , calcium , cord blood , microbiology and biotechnology , ionophore , immunology , biochemistry , antibody , pharmacology , biology , pathology , receptor
We have shown that cultured basophilic cells, derived from human cord blood cells, release histamine and high molecular weight neutrophil chemotactic activity (HMW‐NCA), in a dose‐dependent manner following calcium ionophore and anti‐IgE challenge. The HMW‐NCA detected from human cultured basophilic cells had a similar molecular weight to that of human chopped lung challenged by anti‐IgE as determined by gel filtration chromatography. These results suggest that cultured basophilic cells might be employed for research into the chemical mediators involved in immediate hypersensitivity.