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Detection of Cytomegalovirus‐Specific RNA in Frozen Tissue Sections by Biotin‐Labeled Hybridization Probe
Author(s) -
Eizuru Yoshito,
Mihara Mari,
Minamishima Yoichi,
Tsunematsu Yukiko,
Okabe Nobuhiko
Publication year - 1986
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1986.tb00765.x
Subject(s) - medicine , cytomegalovirus , biotin , spleen , rna , kidney , lung , in situ hybridization , pathology , virology , autopsy , hybridization probe , pneumonitis , virus , antigen , microbiology and biotechnology , dna , herpesviridae , immunology , messenger rna , viral disease , biology , gene , biochemistry
A patient with neuroblastoma developed cytomegalovirus (CMV) pneumonitis and died. At autopsy, CMV was isolated from lung, liver, kidney and submandibular gland. With the biotin‐labeled CMV DNA probe, CMV‐specific RNA was detected in all the organs tested, including the spleen which was negative for virus isolation but positive for CMV‐specific antigens.