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Detection of Rotavirus from Stools by Latex Agglutination Test
Author(s) -
Ushijima Hiroshi,
Shinozaki Tatsuhiko,
Kim Bosu,
Araki Kazuko,
Fujii Ryochi,
Kohno Hideki
Publication year - 1986
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1986.tb00743.x
Subject(s) - rotavirus , latex fixation test , medicine , antiserum , virology , agglutination (biology) , direct agglutination test , reoviridae , microbiology and biotechnology , acute gastroenteritis , antigen , antibody , virus , biology , immunology , serology
Latex agglutination (LA) reagents were prepared using antisera against human rotaviruses (Wa and S2 strains). A commercially available LA test (Rotalex) using antisera against calf rotavirus was also used. Semititrations of rotavirus antigens were compared by three LA tests on 110 stools of children with acute gastroenteritis which had been previously determined rotavirus‐positive by electron microscopy and polyacrylamide gel electrophoresis of genome RNA. LA tests showed diversity of subgroups. The three LA tests reported in this study are concluded to be useful for clinical application in spite of a few exceptions in semititration.