Premium
Effect of Monocytes on Transformation of B‐Lymphocytes by Epstein‐Barr Virus
Author(s) -
Kaiga Kazuhiro
Publication year - 1986
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1986.tb00712.x
Subject(s) - medicine , virus , thymidine , umbilical cord , cord blood , epstein–barr virus , transformation (genetics) , antigen , immunology , virology , microbiology and biotechnology , biology , in vitro , gene , biochemistry
The transformation of human B‐lymphocytes by Epstein‐Barr virus (EBV) was studied in microwell culture by determining 3 H‐thymidine incorporation seven days after infection. B‐lymphocytes were obtained from four human umbilical cord blood samples, four children, and five adults. The level of 3 H‐thymidine incorporation was highest in the cord blood group and lowest in the children, although the difference between each group was statistically not significant for a wide range of individual variations. Correlation between the rate of surface immunoglobulin (SIgM) positive cells and percentage of EBV‐associated nuclear antigen (EBNA) positive cells 24 hrs after infection of 3 H‐thymidine incorporation seven days after infection was not significant. Addition of autologous monocytes suppressed the transformation by EB virus in two cases out of four cord blood specimens, and three cases out of five adult specimens, while it enhanced the transformation in one case out of four child specimens. Also, on clonal transformation by EB virus in agar culture, the effect of addition or monocytes was remarkably suppressive in one of two cases.