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Unusual Lack of the Epitope Recognized by OKT4 on the Helper/Inducer T Lymphocytes
Author(s) -
Fukunaga Yoshitaka,
Ogura Yuichi,
Jinbo Osamu,
Murakami Mutsumi,
Yamamoto Masao,
Ueda Yutaka,
Good Robert A.
Publication year - 1985
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1985.tb00641.x
Subject(s) - monoclonal antibody , population , inducer , antigen , lymphocyte , antibody , immunology , epitope , medicine , peripheral blood mononuclear cell , staining , panning (audio) , t lymphocyte , monoclonal , microbiology and biotechnology , pathology , biology , in vitro , genetics , zoom , paleontology , environmental health , gene , lens (geology)
Lack of the epitope recognized by OKT4 monoclonal antibody on the helper/inducer T lymphocytes in a 14‐year‐old boy with IgA nephritis is described. The lymphocytes reacted normally with OKT3 /Leu4 and OKT8/αLeu2a monoclonal antibodies but not with OKT4 monoclonal antibody. Studies with other monoclonal antibodies (αLeu3a, OKT4A, OKT4B, OKT4C, OKT4D) which also identify the helper/inducer T lymphocyte subset revealed that cells of this population were present in normal numbers among the lymphocytes of the peripheral blood. Staining with OKT4 plus αLeu3a in normal persons indicated that T4 antigen is present on a small population of lymphocytes which lack Leu3a antigen. Further, the intensity of staining of the majority of cells in the subpopulation is increased when these two fluorescienated antibodies are used together. In this patient neither this small OKT4 + Leu 3a ‐ population nor the cells bearing the Leu3a antigen showed OKT4 staining. The findings in the surface marker analysis of E+ OKT8‐ peripheral lymphocytes which were achieved by panning of the patient's peripheral cells indicated the existence of a population of E + OKT8 ‐ peripheral lymphocytes which were achieved by panning of the patient's periphera cells indicated the existence of a population of E + (4A + 4B + 4C4D + )αLeu3al + ly mphocytes in this patient. Lymphocyte responses to PHA, ConA and PWM, however, were all within normal range. Further, this patient had normal serum immunoglobulin levels and exhibited no symptoms or signs of immunodeficiency. These findings indicate that the patient under study has functionally normal helper/inducer T lymphocytes which lack the epitope recognized by OKT4 monoclonal antibody.