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Selective enhancement of human IgE production in vitro by synergy of pokeweed mitogen and mercuric chloride
Author(s) -
Kimata Hajime,
Shinomiya Keisuke,
Mikawa Haruki
Publication year - 1983
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1983.tb01709.x
Subject(s) - immunoglobulin e , pokeweed mitogen , peripheral blood mononuclear cell , medicine , stimulation , in vitro , immunology , antibody , biochemistry , biology
Selective stimulation of IgE production by pokeweed mitogen (PWM) plus mercuric chloride (HgCl 2 ) was further investigated in peripheral blood mononuclear cells (MNC) from non‐atopic donors, who are high and low responders to PWM stimulation with respect to IgG, IgM and IgA production, and from various patients with elevated serum IgE levels. Non‐atopic high responders showed a strong selective increase in IgE plaque forming cells (PFC) without changes in IgG, IgM and IgA PFC, whereas low responders showed a slight increase in IgE PFC only when B cells were co‐cultured with mitomycin C (MMC)‐treated T cells. In patients with elevated serum IgE levels, PWM plus HgCl 2 caused a variable selective increase in IgE PFC, however, there was no correlation between IgE PFC production and serum IgE levels.