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The Effect of Human Gammaglobulin Derivatives on the Human Lymphoblastoid Cell Lines Persistently Infected with Herpes Simplex Virus
Author(s) -
Arita Koji,
Yamauchi Eiko,
Maki Sunao,
Kato Shiro
Publication year - 1981
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1981.tb01642.x
Subject(s) - medicine , virology , herpes simplex virus , gamma globulin , lymphoblast , virus , cell culture , immunology , genetics , antibody , biology
Human lymphoblastoid cells (NC–37) were infected with two strains of herpes simplex virus type 1 (HSV–1). Persistent infections with two strains (a freshly isolated strain, Seike strain, and Miyama strain) of HSV–1 were established in NC–37 cells. In NC–37 cells infected with HSV–1 (Seike), the growth of cells was inhibited,6–72% of viable cells were positive for HSV‐antigen by immuno‐fluorescence, and the percentage of HSV‐antigen positive cells seemed to be inversely related to that of viable cells. Growth of cells and infectious virus were seen for more than 396 days without external support. NC‐37 cells infected with HSV‐1 were subcultured with fresh medium containing human gammaglobulin derivatives. When the percentage of HSV‐antigen positive cells was low (6%), the percentages of HSV‐antigen positive cells decreased to nearly 0% on the 6th day. When the percentage was high (47‐52%), it was not reduced to 0% after one subculture. It was reduced to nearly 0% after three subcultures, and no infectious virus was detected in the treated cells and cultured fluids after more than 16 days. The results of the present study support the speculation that HSV continues to associated with lymphoid cells for a long period of time after appearance of neutralizing antibody, at least for two weeks, and the lymphoid cells infected with HSV have a relation to the pathogenesis of herpetic encephalitis. (Acta Paediatr Jpn 23(3):312–317 1981)