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The Characteristic Profiles of Lysosomal Enzyme Activities in Leukocyte Subpopulations of Normal and Pathologic States
Author(s) -
Tanaka Takeo,
Usui Tomofusa
Publication year - 1981
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1981.tb01247.x
Subject(s) - enzyme , acid phosphatase , lymphocyte , granulocyte , immunology , fucosidase , enzyme assay , medicine , phagocytosis , lysosomal storage disease , biochemistry , biology , glycoprotein , fucose
Summary The levels of lysosomal enzyme activities in human leukocyte subpopulations were examined, using 4‐methylumbelliferyl compounds. There were differences in the composition of the nine enzymes between lymphocytes and granulocytes; the enzyme levels, such as N‐acetyl‐β‐glucosaminidase, β‐galactosidase, β‐fucosidase in lymphocytes and α‐mannosidase, α‐galactosidase, a‐fucosidase in granulocytes, were significantly higher than the other, respectively (p<0.001–0.01). In contrast to cytochemical findings reported by others, the activities examined biochemically in non‐T lymphocytes showed relatively higher than those in T lymphocytes. These lysosomal enzyme levels in some pathologic states were of interest. In I‐cell disease, a low level of β‐galactosidase activity was consistent in lymphocyte and granulocyte of all three cases, but other enzymes showed differences among the cases and between the two types of leukocytes. The enzyme levels in granulocytes and lymphocytes of chronic granulomatous disease showed no significant difference from those of the controls. However, in Chediak‐Higashi syndrome, the activities of α‐mannosidase, α‐galactosidase and α‐fucosidase were low in the patient's granulocytes and the heterozygous granulocytes also showed abnormal enzyme levels such as acid phosphatase, aryl sulphatase and N‐acetyl‐β‐glucosaminidase. The leukemic blast cells showed different enzyme profiles between lymphocytic and myelocytic blast cells. The enzymes, such β‐galactosidase, α‐mannosidase in lymphocytic blasts and N‐acetyl‐β‐glucosaminidase in myelocytic blasts, provided the characteristic profiles of them in each leukemic blast cell.