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The Effect of Human Gammaglobulin Derivatives on Human Lymphoblastoid Cell Lines Persistently Infected with Herpes Simplex Virus
Author(s) -
Arita Koji,
Yamauchi Eiko,
Maki Sunao,
Kato Shiro
Publication year - 1981
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1981.tb00406.x
Subject(s) - virology , herpes simplex virus , antigen , lymphoblast , medicine , virus , antibody , cell culture , in vivo , biology , immunology , microbiology and biotechnology , genetics
Human lymphoblastoid cells (NC‐37) were infected with two strains of herpes simplex virus type 1 (HSV‐1). Persistent infections with two strains (a freshly isolated strain, Seike strain, and Miyama strain) of HSV‐1 were established in NC‐37 cells. In NC‐37 cells infected with HSV‐1 (Seike), the growth of cells was inhibited, 6–72% of viable cells were positive for HSV‐antigen by fluorescent antibody technique, and the percentage of HSV‐antigen positive cells seemed to be inversely related to that of viable cells. Growth of cells and infectious viruses was seen for more than 396 days without external support. NC‐37 cells infected with HSV‐1 were subcultured with fresh medium containing human gammaglobulin derivatives. The percentage of HSV‐antigen positive cells decreased and no infectious viruses were detected in the treated cells and cultured fluids after more than 16 days. It is thought that HSV continues to associate with T‐lymphocytes stimulated in vivo for a long period of time after the appearance of circulating antibody, at least for two weeks, and lymphocytes persistently infect with HSV have a relation to the patho‐genesis of herpesvirus encephalitis in oider children and adults similar to the pathogenesis of SSPE. (Acta Paediatr Jpn 23(2): 201–207 1981)