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Role of the Biliary slgA in the Gastointestinal Tract and the Effect of Malnutrition on the Biliary slgA in the Rat
Author(s) -
Oka T.,
Hosoya N.
Publication year - 1980
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1980.tb00515.x
Subject(s) - biochemistry , biology
The secretory IgA (slgA) was purified from rat bile, and the role of slgA in the intestine and the effect of protein restriction on biliary slgA were investigated in the rat. The purified biliary slgA contained s‐chain and L‐chain and secretory component (SC), and was about 400,000 of molecular weight, about 11.7S. Biliary slgA itself was not digested by trypsin and intestinal fluid, though monomeric IgA (mlgA) was digested. By the challenge of DNP‐BSA, specific antibody against DNP‐BSA was secreted into bile and excreted into feces. Only DNP‐BSA alone was hydrolyzed by trypsin in the presence of biliary slgA containing specific DNP‐BSA antibody. When rats were raised on a diet restricted in protein the concentration of IgA, particularly slgA, in bile and the biliary immune response decreased markedly. These results suggest that biliary slgA involves an important function in the defense of intestinal absorption of foreign antigens such as organisms of infectious diseases and macromolecules in foods, and that the production or the secretion of biliary slgA is sensitively affected by the nutritional situation.