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Inhibition of Lymphocyte Colony Formation in vitro by Culture Supernatant of Human Cord Blood Mononuclear Cells
Author(s) -
Shibasaki Masanao,
Nemoto Hirobumi,
Suzuki Shigeyoshi,
Kuroume Takayoshi
Publication year - 1980
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1980.tb00507.x
Subject(s) - peripheral blood mononuclear cell , concanavalin a , medicine , incubation , in vitro , lymphocyte , cord blood , microbiology and biotechnology , suppressor , immunology , biochemistry , biology , cancer
Culture supernatants from unstimulated cord blood mononuclear cells (CBM) inhibited the proliferation of lymphoid cells of adult and newborns in semisolid cultures and the T‐cell‐rich fraction was found to have mainly this suppressor activity. On the 3rd to 5th day of incubation of CBM, maximal inhibitory activity was found to occur. In contrast, suppressor activity of the culture supernatant was effectively abrogated, when the CBM were incubated for 48 hr with phytohemagglutinin‐P (PHA‐P), concanavalin A (Con A) or protein A added at the concentration of 50 ug/ml or with 1 /ug/m/ of poke‐weed mitogen (PWM) and returned to a mitogen‐free medium for another 48 hr.