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Changes in Lipid Classes and Fatty Acid Compositions in Purified Myelin of EAE Guinea Pig Brain
Author(s) -
Iwaki Nobuhiro
Publication year - 1972
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1972.tb02395.x
Subject(s) - cerebroside , galactolipids , myelin , sphingomyelin , phosphatidyl choline , galactolipid , biochemistry , plasmalogen , ethanolamine , guinea pig , choline , glyceride , fatty acid , medicine , cholesterol , chemistry , phospholipid , endocrinology , central nervous system , membrane , chloroplast , gene
Purified myelin fractions were prepared from brains of control and EAE sensitized guinea pigs on the 5th, 9th and 15th day after injection of antigen. Lipid classes were quantified and expressed as per cent of micromol contents of phospholipids and galactolipids, and fatty acid compositions each of them were analyzed. Cerebroside was markedly reduced with progress of the day, keeping Sulf/Osd ratio in normal constant (0.31–0.36). This was a characteristic finding in this study. Phosphatidyl ethanolamine, ethanolamine plasmalogen and sphingomyelin were also decreasing when compared among phospholipids whereas phosphatidyl choline was increasing. Fatty acid composition showed little change. Anionic lipids like sulfatide are said to play an important role in binding non‐ionic and zwitterionic lipids tightly to the myelin protein. From many reports on electron microscopic observations, it could be considered that the mode of insults to the myelin “lipid” would be rather secondary in demyelination process. The significance of the findings in this study and informations above mentioned were discussed, and a mechanism of demyelination was speculated. In brief, some factors might cause the ionic derangement in the basic amino group of myelin protein, the target of immune reaction, and anionic lipids might therefore be separated from it, being accompanied by such other lipids as cerebroside.