Premium
Membrane Peroxidation and Vitamin E
Author(s) -
Mino Makoto
Publication year - 1971
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1111/j.1442-200x.1971.tb02370.x
Subject(s) - hemolysis , retinol , erythrocyte membrane , vitamin e , vitamin , biochemistry , oxygen , medicine , antioxidant , chemistry , chromatography , membrane , organic chemistry
Summary An examination for the membrane oxidation as a cause of oxygen poisoning was carried out by using erythrocyte membrane. 1) The addition of erythrocytes obtained from healthy human adults to retinol, dissolved in ethanol, resulted in hemolysis. The hemolysis induced by retinol was inhibited by added α‐tocopheryl acetate to the suspensions. 2) The hemolysis was induced by oxygen bubbling to the erythrocyte suspensions. This hemolysis was also inhibited by α‐tocopheryl acetate, while it was accelerated by a previous treatment of the erythrocytes with retinol in a dose of which hemolysis did not occur. The oxygen‐induced hemolysis accelerated with retinol, was also inhibited by α‐tocopheryl acetate. 3) The treatment of the erythrocyte suspensions with H 2 O 2 was carried out in the manner as previously devised by Rose and György, in which the erythrocytes used in this experiment resulted in no hemolysis. When previously treated the erythrocytes with retinol, in a dose of which hemolysis does not occur, hemolysis was induced by adding H 2 O 2 in the same manner, while it was inhibited by α‐tocopheryl acetate. When first treated with H 2 O 2 and subsequently with retinol, no remarkable hemolysis was observed in the erythrocyte suspensions.