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Genetic diversification among cytotypes of Ranunculus silerifolius Lév. (Ranunculaceae)
Author(s) -
MAIDELIZA TESRI,
OKADA HIROSHI
Publication year - 2005
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/j.1442-1984.2005.00130.x
Subject(s) - biology , inbreeding , genetic distance , ranunculus , gene flow , evolutionary biology , locus (genetics) , genetic variation , genetics , intraspecific competition , allele , population , zoology , gene , botany , demography , sociology
Genetic variations of the facultatively autogamous species Ranunculus silerifolius , which show intraspecific chromosomal polymorphisms (Karatsu‐cytotype, Matsuyama‐cytotype, Mugi‐cytotype and Otaru‐cytotype), were investigated in 95 populations using allozymic analysis. Sixteen loci of nine enzyme systems were resolved. Three loci were monomorphic for all populations. On average, 26% of the loci were polymorphic; the 16 loci resolved gave a total of 49 alleles with a mean number of 1.29 alleles per locus. We could not detect any genetic differentiation estimated from allozymic polymorphisms among cytotypes distinguished by chromosome structures in this species. The average genetic distance between populations was large ( D = 0.129), indicating a high level of genetic differentiation and a low level of gene flow ( Nm = 0.171) among the populations, whereas that between cytotypes was very small ( D = 0.008), possibly a result of the relatively short period of time after the occurrence of cytotypes. The overall levels of genetic variation were low as with other facultatively autogamous species. The gene differentiation coefficient among populations, G ST , was high (0.579), which probably reflected the inbreeding in this species. The absence of a correlation between genetic distance and geographic distance may also have resulted from inbreeding.