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Characterization and Evolution of a cDNA Encoding the Small Subunit of Ribulose‐ 1,5‐bisphosphate Carboxylase/ oxygenase ( rbc S) of Stellaria longipes (Caryophyllaceae)
Author(s) -
ZHANG XINGHAI,
MUHAMMAD NASREEN,
CHINNAPPA C.C.
Publication year - 1995
Publication title -
plant species biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 36
eISSN - 1442-1984
pISSN - 0913-557X
DOI - 10.1111/j.1442-1984.1995.tb00119.x
Subject(s) - biology , caryophyllaceae , ribulose 1,5 bisphosphate , genetics , complementary dna , botany , rubisco , gene
A cDNA clone encoding the small subunit of ribulose‐1,5‐bisphosphate carboxylase/oxygenase ( rbc S) of Stellaria longipes (family Caryophyllaceae) was isolated and sequenced. This cDNA was used as a probe to investigate the gene complexity and expression among different genotypes. Southern blot analysis showed that the S. longipes genome contained a rbc S gene family. A higher level of rbc S mRNA was detected in the genotype grown under the long‐day photoperiod and warm condition than under the short‐day photoperiod and cold condition, suggesting the effect of growth condition on the gene expression. Phylogenetic analysis of both the nucleotide and deduced amino acid sequences for both the signal peptide and the mature protein, showed that among ten families of higher plants tested (including four monocot, 14 dicot and one gymnosperm taxa), the rbc S of S. longipes , along with another member of Caryophyllaceae Silene pratensis , was more closely related to the families Solanaceae, Brassicaceae and Fabaceae than it was to other plant families.