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Secretory leukocyte protease inhibitor inhibits neutrophil apoptosis
Author(s) -
SUBRAMANIYAM Devipriya,
HOLLANDER Camilla,
WESTIN Ulla,
ERJEFÄLT Jonas,
STEVENS Tim,
JANCIAUSKIENE Sabina
Publication year - 2011
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/j.1440-1843.2010.01901.x
Subject(s) - slpi , neutrophil elastase , immunology , medicine , chemotaxis , apoptosis , elastase , respiratory tract , inflammation , respiratory system , biology , enzyme , receptor , biochemistry
Background and objective: The secretory leukocyte protease inhibitor (SLPI) is a major anti‐elastase barrier at the epithelial surfaces of upper respiratory tract. In addition to its anti‐protease activity, SLPI has been shown to express anti‐bacterial, anti‐viral and anti‐inflammatory properties. Methods: We measured SLPI concentration in nasal lavage fluid of healthy volunteers after challenge with endotoxin (LPS) and evaluated SLPI effects in vitro on neutrophil chemotaxis, adhesion, cytokine (IL‐8) release and apoptosis. Results: SLPI concentration in nasal lavage ( n = 9) 2, 6 and 24 h after the challenge with LPS (25 µg) increased from 32% to 238% compared with baseline (226 ± 71 ng/mL). In vitro , SLPI (20–80 µg/mL) induced neutrophil chemotaxis (sixfold, P < 0.001) and decreased neutrophil apoptosis by 73% ( P = 0.006), relative to controls. However, SLPI had no affect on IL‐8 release or neutrophil adhesion to fibronectin. SLPI‐positive immunoreactivity was co‐localized with neutrophils in lung specimens from patients with COPD. Conclusions: Our findings indicate upregulation of SLPI in response to LPS in nasal secretions and show anti‐apoptotic effects of SLPI in primary human neutrophils suggesting a new role of SLPI during neutrophilic inflammation.