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Carbocisteine can scavenge reactive oxygen species in vitro
Author(s) -
NOGAWA Hisashi,
ISHIBASHI Yuji,
OGAWA Akitsu,
MASUDA Kayoko,
TSUBUKI Takeshi,
KAMEDA Tomoko,
MATSUZAWA Shigeki
Publication year - 2009
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/j.1440-1843.2008.01424.x
Subject(s) - reactive oxygen species , peroxynitrite , hypochlorous acid , oxidative stress , free radical scavenger , intracellular , pharmacology , hydrogen peroxide , reactive nitrogen species , biochemistry , scavenger , hydroxyl radical , medicine , microbiology and biotechnology , chemistry , antioxidant , superoxide , biology , enzyme
Background and objective: Reactive oxygen species (ROS) play an important role in the pathogenesis of various respiratory diseases. Carbocisteine, a mucoregulatory drug, is used in the treatment of several disease states but little information is available about its scavenger effects on ROS. The present study was designed to examine the scavenger effects of carbocisteine on ROS. Methods: The oxidation‐reduction potential of carbocisteine was measured, and its scavenger effects on hypochlorous acid (HOCl), hydrogen peroxide (H 2 O 2 ), hydroxyl radical (OH • ) and peroxynitrite (ONOO ‐ ) were examined in cell‐free conditions. The effects of carbocisteine on ROS generated from rat neutrophils, intracellular oxidative stress and release of inflammatory cytokines (IL‐8 and IL‐6) from IL‐1β‐induced airway epithelial cells, NCI‐H292 cells, were investigated. Results: Carbocisteine provided a reducing stage and showed scavenger effects on H 2 O 2 , HOCl, OH • and ONOO ‐ in cell‐free conditions. Carbocisteine inhibited ROS generation from rat neutrophils, intracellular oxidative stress and release of IL‐8 and IL‐6 from NCI‐H292 cells. N ‐acetyl‐ l ‐cysteine, a radical scavenger, also inhibited these events related to ROS as well as carbocisteine. Conclusions: These results suggest that carbocisteine could exert anti‐inflammatory and anti‐oxidant effects through directly scavenging ROS in addition to its previously known mucoregulatory effect.