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New quinolone, grepafloxacin, inhibits Cl − secretion across bovine airway epithelium in culture
Author(s) -
Kanoh Soichiro,
Tamaoki Jun,
Kondo Mitsuko,
Nagai Atsushi
Publication year - 2001
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/j.1440-1843.2001.00296.x
Subject(s) - amiloride , secretion , epithelium , channel blocker , respiratory epithelium , transepithelial potential difference , medicine , ion transporter , pharmacology , epithelial sodium channel , biophysics , biochemistry , chemistry , biology , sodium , pathology , membrane , organic chemistry , calcium
Objective: Transepithelial ion transport plays an important role in the regulation of the amount and the rheological properties of bronchial secretion. The effect of grepafloxacin (GPFX), a new quinolone agent, on bioelectrical properties of airway epithelium was determined. Methodology: Electrical properties of bovine tracheal epithelium cultured under an air–liquid interface condition were measured by the short‐circuit technique. Results: Addition of GPFX (100 μg/mL) to the mucosal side decreased short‐circuit current (Isc) from 14.4 ± 1.3 to 5.6 ± 0.6 μA/cm 2 ( P < 0.001), and the response was accompanied by corresponding decreases in transepithelial potential difference and cell conductance. This effect was concentration dependent, and a similar response was also noted when GPFX was added to the submucosal side. The GPFX‐induced decrease in Isc was not altered by the Na + channel blocker amiloride, but was inhibited by the Cl − channel blocker diphenylamine‐2‐carboxylate or Cl − ‐free medium ( P < 0.001, in each case). Furthermore, GPFX reduced Cl − conductance ( P < 0.01) without affecting Na + conductance of the epithelium. Conclusions: Grepafloxacin selectively inhibits Cl − secretion across tracheal epithelial cells, which may result in the inhibition of water secretion and, hence, the reduction of airway secretion.