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Determination of adenosine deaminase activity and its isoenzymes for diagnosis of pleural effusions *
Author(s) -
Gorguner Metin,
Cerci Melek,
Gorguner Ilknur
Publication year - 2000
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/j.1440-1843.2000.00270.x
Subject(s) - adenosine deaminase , medicine , effusion , tuberculosis , gastroenterology , pleural effusion , etiology , parapneumonic effusion , respiratory disease , pleural disease , pleural fluid , pathology , adenosine , surgery , lung
Objective: We aimed to investigate adenosine deaminase (ADA) activity and the activities of its ADA 1 and ADA 2 isoenzymes in pleural effusions and also sera with different aetiological origins. Methodology : The pleural effusions of 87 patients were examined. The patients were separated into four groups: transudates, parapneumonic, malignant, and tuberculous effusions. The cases were also designated as tuberculous or non‐tuberculous group. Adenosine deaminase activity was determined by the colorimetric method described by Giusti and Galanti. Results: The intermean differences were statistically significant for total ADA, ADA 1 and ADA 2 , except for pleural fluid ADA 1 in the malignant group when compared to the tuberculous effusion group. The intermean differences between the tuberculous and non‐tuberculous group were statistically significant for all three parameters except for pleural fluid and serum ADA 1 activity. The sensitivities of total ADA, ADA 1 and ADA 2 activities for tuberculosis were 91, 57 and 93%, respectively; their specificities 89, 88 and 92%, respectively; their positive predictive values 82, 70 and 86%; and their diagnostic accuracies 89, 76 and 92%, respectively, in pleural fluid. Conclusions: Determination of ADA and its isoenzymes can help to differentiate the causes of pleural effusion. Increased ADA 2 activity is a striking marker of tuberculous effusions. In contrast, increased ADA 1 activity was significantly elevated in parapneumonic effusions.