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Possible surface carbohydrates involved in signaling during conjugation process in Zygnema cruciatum monitored with fluorescein isothiocyanate‐lectins (Zygnemataceae, Chlorophyta)
Author(s) -
Kim Gwang Hoon,
Yoon Minchul,
West John A.,
Klochkova Tatyana A.,
Kim SungHo
Publication year - 2007
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/j.1440-1835.2007.00456.x
Subject(s) - agglutinin , biology , lectin , soybean agglutinin , fluorescein isothiocyanate , isothiocyanate , wheat germ agglutinin , concanavalin a , peanut agglutinin , fluorescein , biochemistry , biophysics , fluorescence , physics , quantum mechanics , in vitro
SUMMARY The changes of cell surface carbohydrates were examined with FITC (fluorescein isothiocyanate)‐labeled lectins during the conjugation process of the green alga Zygnema cruciatum . The Ulex europaeus agglutinin (UEA)‐specific materials were detected consistently on the surface of vegetative cells, but were absent on the surface of protruding papillae or conjugation tube. The tips of male and female papillae were labeled with soybean agglutinin (SBA) and peanut agglutinin (PNA) during conjugation. The SBA‐ and PNA‐specific materials appeared first at the tip of male papillae and began to accumulate on the surface of female papillae. No labeling of these lectins was detected on the surface of vegetative filaments throughout the conjugation process. FITC‐ConA (Concanavalin A) and FITC‐RCA ( Ricinus communis agglutinin) did not label the vegetative filaments of Z. cruciatum , but a trace labeling of these lectins was observed on the surface of some swollen papillae occasionally. Blocking experiments with various lectins showed that these SBA‐ and PNA‐specific glycoconjugates might be involved in the signaling between male and female papillae.