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Isolation of organellar DNA from Codium fragile (Codiaceae, Codiales, Ulvophyceae)
Author(s) -
Satoh Masaya,
Sakai Atsushi,
Hamazaki Shizue,
Takashima Yuki,
Kuroiwa Tsuneyoshi
Publication year - 1997
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/j.1440-1835.1997.tb00078.x
Subject(s) - biology , mitochondrial dna , nuclear dna , restriction enzyme , dna , chloroplast dna , ecori , microbiology and biotechnology , chloroplast , genetics , gene
SUMMARY Organellar DNA was isolated from Codium fragile (Suringar) Hariot (Codiaceae, Codiales, Ulvophyceae) by CsCI‐buoyant density centrifugation in the presence of Hoechst dye 33258. Three bands were formed by ultracentrifugation and each fraction of DNA was identified by Southern hybridization. The uppermost fraction was identified as chloroplast DNA, the middle fraction was nuclear DNA and the bottom fraction was mitochondrial DNA. Nuclear rDNA was isolated in the same fraction as mitochondrial DNA. The estimated genome size of mitochondrial DNA by analysis with restriction endonucleases was more than 141.6 kb, which was larger than that of microalgae but smaller than land plants. Restriction endonuclease analysis of the chloroplast DNA showed no difference with that known of C. fragile in New York.