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Cytomorphogenesis in cenocytic green algae. V. Segregative cell division and cortical microtubules in Dictyosphaeria cavernosa (Siphonocladales, Chlorophyceae) *
Author(s) -
Okuda Kazuo,
Mine Ichiro,
Morinaga Takeshi,
Kuwaki Norie
Publication year - 1997
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/j.1440-1835.1997.tb00075.x
Subject(s) - protoplasm , biology , cell division , microtubule , cytoplasm , botany , cytoplasmic streaming , anatomy , biophysics , microbiology and biotechnology , cell , biochemistry
SUMMARY Disassembly and reassembly of cortical microtubules (MT) during and after segregative cell division (SCO) in Dictyosphaeria cavernosa (Forssk.) Børgesen were observed using fluorescence microscopy. Parallel cortical MT in a mother cell were intact just after the initiation of SCD, but soon circular, MT‐free patches appeared. Protoplasmic contraction enlarged the patches, and in these areas, the protoplasm eventually became perforated. Long and undulating cortical MT were arranged densely in the reticulate protoplasm. During further protoplasmic contraction, cortical MT appeared to be random and decreased in density. Finally, short and random cortical MT were present in the segregated protoplasts. Parallel cortical MT reassembled in the expanding daughter cells. After the daughter cells came in contact with one another, a radial system of cortical MT was constructed at the side that faced the inside of the mother cell wall. A microtubule inhibitor (amiprophos methyl, APM) had no effect on SCO. Segregative cell division was not induced directly by mechanical wounding. A comparison between SCO and wound‐induced protoplasmic contraction was made.